About CSI 3.0:

CSI 3.0 web server is designed to identify 11 types of secondary and supersecondary structures. In particular, CSI 3.0 uses backbone NMR chemical shifts (Cα, CO, Cβ, N, Hα, NH) and sequence data to identify α helices, β strands, β turns (type I, II, VIII, I′ and II′), coil regions, β hairpins, interior β strands and edge β strands. CSI 3.0 accepts either a BMRB (BioMagResBank) formatted (NMR-STAR 2.1 or NMR-STAR 3.1) or a SHIFTY formatted file with at least 70-80% of the backbone atoms having chemical shifts assigned. The server generates a text-based secondary structure assignment as well as a colorful CSI plot (bar graphs) for small-medium sized proteins in approximately 2-5 minutes (depending on the size of proteins and the server load). If you use CSI 3.0 please cite the following reference:

Reference: Noor E. Hafsa, David Arndt, David S. Wishart- CSI 3.0: a web server for identifying secondary and super-secondary structure in proteins using NMR chemical shifts, Nucleic Acids Research (2015): Vol. 43, Issue W1, p. W370-W377


CSI 3.0 Input/Output Gallery

CSI 3.0 Sample Assessment of 13 Selected Proteins: Excel file
CSI 3.0 Sample Assessment of 10 Selected Proteins: Excel file, Summary

CSI 3.0 Usage

Upload file with chemical shifts here:


Paste chemical shifts into the text box below:

CSI 3.0 accepts BMRB NMR-STAR 2.1 and 3.1 formats, and SHIFTY format.
See format requirements and examples.

(NOTE: If a file is selected for upload above, it will be used and content of the text box below will be ignored)




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